The inulin hydrolysis by recombinant exo-inulinases: determination the optimum temperatures and activation energies

Abstract The advantages of recombinant enzymes over native include the control in a production environment, product purity and also high yield. paper presents determination optimum temperatures activation energies for various origin exo-inulinases, among others from Aspergillus niger , A. awamori Kluyveromyces marxianus K. cicerisporus . parameters were estimated based on literature activity curves versus temperature hydrolysis inulin. It was assumed that both reaction process deactivation exo-inulinase first-order reactions by enzyme concentration. A mathematical model describing effect used. Based comparison analysis, values $${E_{\rm }}$$ E a range $${32.01 \pm 7.80}$$ 32.01 ± 7.80 to $${43.83 4.87}$$ 43.83 4.87 kJ mol $$^{-1}$$ - 1 d d $${83.93 4.82}$$ 83.93 4.82 $${352.44 14.26}$$ 352.44 14.26 $${T_{\rm opt T opt obtained $${318.91 }$$ 318.91 1.19 $${328.76 0.25}$$ 328.76 0.25